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Lytic reagents used on clinical blood samples to lyse non-nucleated red blood cells and preserve white blood cells in order to perform white blood cell counts and the quantitative determination of hemoglobin.
Mouse Lymph Nodes paraffin sections are made of freshly harvested tissues, fixed in 10% neutral buffered formalin, and processed for paraffin embedding. Paraffin?blocks are sectioned at a thickness of 5microns and mounted on positively charged slides (2-4 sections per slide depending on tissue size).
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A mixture of bioactive sphingolipids; found in mammalian cell membranes; make up 2-15% of the total organ phospholipid population but are found at higher concentrations in the brain and myelin sheaths surrounding peripheral nerves; interact with cholesterol to control its distribution within cellular membranes and maintain cholesterol homeostasis in cells; undergo hydrolysis by sphingomyelinase to form ceramides
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OptiLyse B is an erythrolytic reagent for lysing human red blood cells following direct immunofluorescence staining of whole blood for flow cytometry. OptiLyse B is a ready-to-use lysing solution optimized for use on BD Biosciences instruments.
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Mouse Lymph nodes total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of 6 mammalian protease inhibitors to minimize proteolysis, precisely quantified, and stored at -80oC.
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